Molecular biological analysis of the pathogen of respiratory tract infection caused by human adenovirus 7

CAO Mengchan; SUN Yong; XU Siqing; LI Xianxiang; LIU Minhong

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Anhui Journal of Preventive Medicine ›› 2019, Vol. 25 ›› Issue (2) : 81-83.

Molecular biological analysis of the pathogen of respiratory tract infection caused by human adenovirus 7

CAO Mengchan¹, SUN Yong², XU Siqing¹, LI Xianxiang¹, LIU Minhong¹

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Abstract

Objective An outbreak of acute respiratory tract infection caused by human adenovirus type 7 in an army of Anqing City was identified by etiology, and the Hexon gene was sequenced and analyzed. Methods Thirteen throat swabsamples were collected by real-time PCR for adenoviral nucleic acid detection. The positive samples were inoculated with Hep-2 cells for virus isolation and culture.The positive isolated cultures were collected and sequenced by RT-PCR to amplify the nucleotide sequence of Hexon.The obtained sequences were subjected to homologous alignment in the NCBI GenBank database, and the evolutionary tree was constructed by MAGE6.0 software. Results Ten positive strain cultures were obtained. The Hexon gene sequence was 100% homologous with the epidemic strain Z9/WH/CHN/2015 of adenovirus type 7 in Wuhan, China (GenBank No.KX897164), and 100% homologous with the epidemic strain of AQHAdv7 in 2012 and the sporadic strain of AQHAdv7 in 2015. Conclusion The epidemic was caused by human adenovirus type 7 infection. The genotype of the adenovirus epidemic strains prevalent in Anqing was stable. The epidemic may have originated from the local epidemic strains.

Key words

Human adenovirus / Gene sequence / Acute respiratory tract infection / Evolutionary analysis

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CAO Mengchan , SUN Yong, XU Siqing, LI Xianxiang, LIU Minhong. Molecular biological analysis of the pathogen of respiratory tract infection caused by human adenovirus 7[J]. Anhui Journal of Preventive Medicine. 2019, 25(2): 81-83

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