Molecular typing and genetic characterization of norovirus in two outbreaks in Huangshan City in 2018

CHENG Peng; SHI Yonglin; YANG Xiaoshuang; ZHANG Zhongxin

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Anhui Journal of Preventive Medicine ›› 2019, Vol. 25 ›› Issue (3) : 174-177.

Molecular typing and genetic characterization of norovirus in two outbreaks in Huangshan City in 2018

CHENG Peng¹, SHI Yonglin², YANG Xiaoshuang¹, ZHANG Zhongxin³

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Abstract

Objective To understand the molecular typing and genetic characteristics of norovirus in the outbreak in Huangshan City. Methods Thirty-seven anal swabs from patients with gastroenteritis in two outbreaks in Huangshan City in 2018 were collected.Norovirus nucleic acid was detected by real-time fluorescence quantitative polymerase chain reaction (Real-time PCR).The positive samples were detected.The capsid and polymerase regions of the virus were amplified by reverse transcription-polymerase chain reaction (RT-PCR), and the products of the PCR were analyzed.The PCR products were sequenced and identified by molecular typing.The sequencing results were analyzed by using Mega 6.0 software to construct phylogenetic tree. Results Of the 37 samples, 16 were positive for norovirus nucleic acid, of which 11 were successfully sequenced.BLAST analysis showed that 11 sequences were genotypes of norovirus GII.P16-GII.2.The sequence of norovirus detected in the two outbreaks had high homology with that of Quanzhou City in Fujian Province, Taizhou and Nanjing City in Jiangsu Province, and Suzhou City in Anhui Province. Conclusion GII.P16-GII.2 Norovirus was the main pathogen causing two outbreaks of acute gastroenteritis in Yixian County,Huangshan City.

Key words

Norovirus / Molecular typing / Evolutionary analysis / Outbreaks

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CHENG Peng, SHI Yonglin, YANG Xiaoshuang, ZHANG Zhongxin. Molecular typing and genetic characterization of norovirus in two outbreaks in Huangshan City in 2018[J]. Anhui Journal of Preventive Medicine. 2019, 25(3): 174-177

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