Objective To evaluate the clinical application performance of a domestic human immunodeficiency virus type 1 (HIV-1) nucleic acid detection reagent. Methods A total of 209 clinical samples were collected and tested simultaneously with the HIV quantitative nucleic acid detection kit of Mérieux Company and Northeast Pharmaceutical Group.The relationship between the data obtained by the two reagents was analyzed.The consistency of the two reagents was analyzed by Kappa test, and the correlation between the two reagents was analyzed by Bland-Altman test and paired t test. Results The Kappa detection value of Northeast Pharmaceutical Group and Merier Company was 1.00, so the overall coincidence rate of the two companies was 100%.The correlation analysis of the quantitative test results of the two kits showed that the correlation between the two kits was high, and the test results were identical.The paired t test showed that the two kits were equivalent. Conclusion The results of HIV quantitative nucleic acid test kit of Northeast Pharmaceutical Group and HIV test kit of Mérieux Company for the same clinical sample are qualitatively consistent.At the same time, the quantitative results have a high degree of correlation and consistency.