Objective To analyze the follow-up referral the situation of referral tracking of suspected tuberculosis (TB) patients reported by non-tuberculosis prevention and control institutions in Huangshan City from 2014 to 2018, find out the weak links in the follow-up referral work of suspected tuberculosis patients, and take corresponding measures to improve the discovery detection level of tuberculosis patients. Methods Data on referral, tracing, arrival and diagnosis of suspected tuberculosis patients reported by non-tuberculosis prevention institutions in Huangshan TB Information Management System from 2014 to 2018 were collected.The referral arrival rate, tracing arrival rate, overall arrival rate, detection rate of arrived patients with active pulmonary tuberculosisactive pulmonary tuberculosis of arrived patients, exclusion rate of arrived patients with active pulmonary tuberculosisactive pulmonary tuberculosis of arrived patients, and contribution rate of non-tuberculosis prevention agencies institutions to for tuberculosis and smear-positive tuberculosis in five years were statistically analyzed.The contribution rate was analyzed. Results From 2014 to 2018, the referral arrival rate in Huangshan City decreased from 40.13% to 29.39%; The tracking arrival rate increased from 76.94% to 92.39%; The overall arrival rate increased from 86.59% to 95.47%. The detection rate of active pulmonary tuberculosis in place decreased from 55.36% to 49.85%; The exclusion rate increased from 38.92% to 45.21%. The contribution rate of active pulmonary tuberculosis and smear-positive pulmonary tuberculosis found in non-TB prevention institutions was relatively stable, and the average level was still higher than 60% in five years. Conclusion Medical and preventive cooperation should continue to be strengthened. By increasing the publicity of public tuberculosis knowledge and the training of clinicians, the quality of the network direct reporting information system and the level of discovery of tuberculosis patients will be improved.We should continue to strengthen medical and preventive cooperation, enhance the quality of the work of the network direct reporting information system and improve the level of detection of tuberculosis patients by increasing the publicity of tuberculosis knowledge among the public and the training of clinicians.