Objective To understand the present situation of disinfection effect in nursery institutions in Jiujiang District of Wuhu City, find out the problems existing in the disinfection management of nursery institutions, and guide nursery institutions to better carry out disinfection work. Methods The indoor air, toys, surface of objects, hands of children and teachers, tableware and ultraviolet radiation of all nursery institutions filed by the Education Bureau in the whole district were detected by spot checking method.The samples for random inspection were tested and the qualified situation was analyzed. Results In the past seven years, 728 samples of nursery institutions in Jiujiang District of Wuhu City were tested, 416 of which were qualified,the qualified rate was 57.1%.The lowest qualified rate was 30.7% in 2018, and the highest was 77.8% in 2017.The difference was statistically significant (χ²= 44.464,P＜0.01).The sample qualified rate of samples of nursery institutions in Jiangnan part and Jiangbei part was 63.2% and 49.0%, respectively.There was statistical significance (χ²= 14.644, P＜0.01).Among the seven kinds of samples, the highest qualified rate was tableware (84.0%) and the lowest was hand sample of children (33.1%).The difference was statistically significant (χ²= 80.375, P＜ 0.01).The qualified rates of public and private nursery institutions were 69.9% and 55.5%, respectively, with statistical significance(χ²=6.205, P=0.013). Conclusion The disinfection quality of nursery institutions in Jiujiang District is not high, and the qualified rate of all kinds of samples except tableware is not high.The relevant departments should strengthen management to further improve the disinfection quality of nursery institutions.