Objective To investigate the causes and control strategies of norovirus infection outbreak in a kindergarten in Huaining County through field investigation and disposal. Methods Field epidemiological investigation was used to investigate the cases of norovirus infection outbreak in a kindergarten in Huaining County.The samples of cases and food samples were collected and the nucleic acid of norovirus was detected by fluorescence quantitative-polymerase chain reaction. Results A total of 32 cases of norovirus infection were reported in a kindergarten in Huaining County.The incidence rate was 26.67%(32/120).The cases were mainly distributed in the class where the first case occurred.The epidemic lasted for three days.There was no significant difference between male and female (χ²=0.13,P= 0.719).Among 11 anal swabs, 7 were positive for norovirus nucleic acid, and all of them were G-II, with a positive rate of 63.64%.4 anal swabs from close contacters and 6 food samples were negative for norovirus nucleic acid. Conclusion The outbreak was caused by Norovirus type G-II infection, and the main cause of the outbreak was that the children did not take effective isolation and failed to deal with the vomit in the classroom in time.Close contact with the vomit aerosol and human-to-human transmission may be the main cause of the outbreak.